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KMID : 0613320110170010023
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Journal of the Korean Bone and Joint Tumor Soceity
2011 Volume.17 No. 1 p.23 ~ p.29
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Over-expression of PTEN Involved in Troglitazone-induced Apoptosis in Human Osteosarcoma Cells
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Yoon Sun-Jung
Zhou Lu
Kim Jung-Ryul
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Abstract
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Purpose:We investigated the effects of phosphatase and tensin homologue deleted on chromosome 10 gene phosphatase and tensin homologue deleted on chromosome 10 gene (PTEN) expression on the cell proliferation and on the responsiveness of troglitazone in osteosarcoma cells.
Materials and Methods :Western blotting alnalysis was performed to detect the expression of PTEN in U-2OS cells treated with troglitazone. WST (water-soluble tetrazolium) assay was used to evaluate cell proliferation. Flow cytometry was used to determine cell apoptosis. Further, transfection of wild-type PTEN plasmid DNA was used to upregulate PTEN expression.
Results:Troglitazone treatment induced growth inhibition of U2-OS cells in a dose- and time-dependent manner. Troglitazone increased the expression of PTEN in a dose-dependent manner. PTEN upregulation induced by troglitazone treatment resulted in cell growth inhibition and apoptosis in U-2OS cells. PTEN over-expression by plasmid transfection enhanced these effects of troglitazone. Moreover, no changes were observed in the mutant type-PTEN group.
Conclusion:Upregulation of PTEN is involved in the inhibition of cell growth and induction of cell apoptosis by troglitazone. Further, PTEN over-expression can cause cell growth inhibition in osteosarcoma cells and these cell growth inhibitions could be enhance by troglitazone treatment.
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KEYWORD
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osteosarcoma, apoptosis, PTEN (phosphatase and tensin homologue deleted on chromosome 10 gene), PPARγ (peroxisome proliferator-activated receptor γ) agonist, troglitazone
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